TY - RPRT TI - Tau assemblies enter the cytosol in a cholesterol sensitive process essential to seeded aggregation AU - Tuck, Benjamin J. AU - Miller, Lauren V. C. AU - Wilson, Emma L. AU - Katsinelos, Taxiarchis AU - Cheng, Shi AU - Vaysburd, Marina AU - Knox, Claire AU - Tredgett, Lucy AU - Metzakopian, Emmanouil AU - James, Leo C. AU - McEwan, William A. AB - Abstract Accumulating evidence supports a prion-like mechanism in the spread of assembled tau in neurodegenerative diseases. Prion-like spread is proposed to require the transit of tau assemblies to the interior of neurons in order to seed aggregation of native, cytosolic tau. This process is poorly understood and remains largely hypothetical. Here, we develop sensitive techniques to quantify the cytosolic entry of tau in real-time. We find that tau does not promote its own entry but, rather, is wholly dependent on cellular machinery. We find that entry to the widely used reporter cell line HEK293 requires clathrin whereas entry to neurons does not. Cholesterol depletion or knockdown of cholesterol transport protein Niemann-Pick type C1 in neurons renders cells highly vulnerable to cytosolic entry and seeded aggregation. Our findings establish entry as the rate-limiting step in seeded aggregation and demonstrate that dysregulated cholesterol, a feature of several neurodegenerative diseases, potentiates tau aggregation. Graphical Abstract DA - 2021/06/22/ PY - 2021 DP - DOI.org (Crossref) LA - en M3 - preprint PB - Cell Biology UR - https://biorxiv.org/lookup/doi/10.1101/2021.06.21.449238 Y2 - 2022/02/17/14:02:21 KW - preprint ER -